Amazon UK and Argos have stayed at last year's number one and two spots respectively, while Tesco has knocked Play.com off the number three spot, moving it down one place to number four.
Experian said that one of the fast moving sectors were department stores and John Lewis was one of those, moving up one place and into the top ten.
Supermarkets were also fast movers, but despite the main site's success, Tesco Direct slipped down two places to number 11. Sainsbury's meanwhile shot up the charts from number 39 up to number 22. Asda also climbed up from number 28 to number 24.
Relative newcomer, Very.co.uk, fell from last year's position 33, to number 43.
Robin Goad, director of research, Experian Hitwise commented:"This has been another fascinating year for online retail and looking at the churn of websites in the Hot 100 list just goes to show how competitive the online space has become.
"We've seen some pretty significant movement within the top ten online retailers, with Tesco moving up to third place and B&Q breaking into the top 10 for the first time. Higher ranking in the Hot 100 means more visits coming to a brand's website which is a high priority for most retailers.
"Some companies are choosing to increase their online presence in order to increase the reach of their brand, whilst others are using online as a customer service tool, but ultimately more visits to a website means more sales and it is this reason that is driving the thriving competition between our Hot 100 retailers.
"The key trends we've witnessed this year are that department stores and fashion retailers are expanding their online capabilities in order to drive more sales."
For the full Hot 100 list, simply click here.