The new limited edition range is based on the online game and is available for pre-order today.
Best Buy will offer eight exclusive FarmVille plush toys in store and online at BestBuy.com this Christmas.
Characters include a pig, goat, cow, sheep, chicken, duck, horse and rabbit, and each is dressed in seasonal winter clothing.
The plush will retail at $9.99, and come with ten free Farm Cash and a digital code that can be used in the online game to collect a limited-edition, in-game version of the plush toy. The toys are packaged in a festive, red mystery box.
The toys also will be available as part of a game card bundle. When children purchase a $25 game card and receive one plush for 99 cents.
"At Best Buy we know how much our customers enjoy FarmVille and we're thrilled to offer them this first-of-its kind product, just in time for the holidays," said Alix Hart, senior director, digital marketing at Best Buy.
"By collaborating with Zynga, FarmVille fans will be able to bring their favourite virtual characters home to enjoy throughout the season."
"A top request we've heard from our players has been for real toys that mirror our great FarmVille animals," said Ryan Linton, senior director, business development at Zynga. "We've listened, and with the help of Best Buy, we've brought these great characters to life."