Proprietor Alan Hogston has made the decision not to renew the lease on the store due to the decline of shoppers in the area.
He said: "When the lease comes to an end, the rent automatically goes up and we just don't get the number of people down Desborough Road to make it a viable shop anymore.
"Desborough doesn't get the people down there anymore, that's the problem. It has been on the decline for about three years."
Despite having to close one shop down, Hogston remains positive and has plans to improve his Hazlemere store and will be on the lookout for new premises to replace the Desborough branch.
He added: "You have got to stay positive. New beginnings - not end of an era."